Stem cell therapy for spinal cord injury: The Spinal Cord Journey

A short film about three spinal cord injury patients who share their stories of tragedy, hope and recovery following stem cell treatments for spinal cord injury at the Stem Cell Institute in Panama City, Panama. See them demonstrate their improvements by lifting themselves up, standing and even walking after stem cell therapy.

The dual effect of MSCs on tumour growth and tumour angiogenesis

Michelle Kéramidas, Florence de Fraipont, Anastassia Karageorgis, Anaïck Moisan, Virginie Persoons, Marie-Jeanne Richard, Jean-Luc Coll and Claire Rome

Abstract (provisional)
Introduction

Understanding the multiple biological functions played by human mesenchymal stem cells (hMSCs) as well as their development as therapeutics in regenerative medicine or in cancer treatment are major fields of research. Indeed, it has been established that hMSCs play a central role in the pathogenesis and progression of tumours, but their impact on tumour growth remains controversial.

Our results suggest that hMSCs injection decreased solid tumour growth in mice and modified tumour vasculature, which confirms hMSCs could be interesting to use for the treatment of pre-established tumours.

Methods

In this study, we investigated the influence of hMSCs on the growth of pre-established tumours. We engrafted nude mice with luciferase-positive mouse adenocarcinoma cells (TSA-Luc+) to obtain subcutaneous or lung tumours. When tumour presence was confirmed by non-invasive bioluminescence imaging, hMSCs were injected into the periphery of the SC tumours or delivered by systemic intravenous injection in mice bearing either SC tumours or lung metastasis.

Results

Regardless of the tumour model and mode of hMSC injection, hMSC administration was always associated with decreased tumour growth due to an inhibition of tumour cell proliferation, likely resulting from deep modifications of the tumour angiogenesis. Indeed, we established that although hMSCs can induce the formation of new blood vessels in a non-tumoural cellulose sponge model in mice, they do not modify the overall amount of haemoglobin delivered into the SC tumours or lung metastasis. We observed that these tumour vessels were reduced in number but were longer.

Conclusions

Our results suggest that hMSCs injection decreased solid tumour growth in mice and modified tumour vasculature, which confirms hMSCs could be interesting to use for the treatment of pre-established tumours.

Original Link: http://stemcellres.com/content/4/2/41/abstract

Stem Cell Therapy for Heart Failure – Lillian Rowland

Stem Cell Therapy for Heart Failure patient Lillian RowlandLillian Rowland is a 79 year-old [former] heart failure patient from Ohio. She was diagnosed with heart failure in March 2012. Her left ventricular ejection fraction (LVEF) at that time was measured at 25%. Normal LVEF range is 55% – 70%.

Lillian’s cardiologist recommended an implantable defibrillator. At the time, she did not want to go through the implantation procedure so she declined. Lillian decided to go to the Stem Cell Institute (SCI) in Panama for human umbilical cord-derived stem cell treatments after hearing about it from her son Jay Lenner who works for SCI as its Public Relations Manager.

Below is a brief interview with Lillian in March 2013. Today, her heart is back into normal range (LVEF = 55%) and her cardiologist told her that she no longer needs an implantable defibrillator.

What was your diagnosis?

I was diagnosed with heart failure.

When were you diagnosed?

I was diagnosed in March 2012.

What symptoms did you have?

I couldn’t breathe at night. I was sleeping and I had to get up. I was gasping for breath.

Why did you choose the Stem Cell Institute?

Because my son works in stem cells and after I got out of the hospital in Arizona he wanted to take me down to have stem cells to see if it could help repair the damage to my heart.

How where the doctors at the clinic?

The doctors were very nice and there’s really nothing to it. It’s just an injection that goes into [a catheter placed into a vein inside] your hand. It’s not like they are operating on you or anything.

How was the clinic?

The clinic is very clean and sterile and the people are very nice.

Do you have any symptoms now?

It’s been a year since I had the stem cells and I am symptom free.

I had an echocardiogram a year after the stem cells and when they read it my ejection fraction was 55%. They wanted to put a defibrillator in me and the heart doctor came up and said, there’s no reason to put one in now because my heart was OK.

Would you recommend that other heart failure patients go to Panama for treatment?

Yes I would.

Do you think this changed your life?

Yes. I don’t have to worry about having a heart problem!

The umbilical cord stem cells we use are recovered from donated umbilical cords following normal, healthy births. Before they are approved for use in treatments, all umbilical cord-derived stem cell samples are screened for viruses and bacteria to International Blood Bank standards.

Stem Cell Therapy for Heart Disease – Jim Parker Attorney at Law and former Texas State Legislature Member

Stem Cell Therapy Heart Patient Jim ParkerJim Parker, Attorney at Law is a former Texas State Legislature member who was treated with umbilical-cord derived stem cells for heart disease at the Stem Cell Institute in Panama in March 2011.

Jim was kind enough to answer some questions about his condition before and after treatment. He also discusses his trip to Panama, the clinic and its staff.

What is your diagnosis and when were you diagnosed?

Exactly what my diagnosis was I cannot say in medical terms. Factually speaking, I had five (5) by-passes in 1995 (or thereabouts) and then had nine (9) stents added over the next 13 or 14 years. I began to experience severe angina in January and February of 2011 and was hospitalized in February with a series of heart attacks. I had consulted my cardiologist in Abilene and had also gone to a cardiologist in Dallas who was supposed to be really good. They both told me that there was simply nothing else I could do. When I was released from the local hospital in early March of 2011, my family doctor gave me a bottle of morphine and a bottle of nitroglycerine and basically told me to make it as well as I could for as long as I could.

Panama did not exactly change my life. It literally gave my life back to me.

We had heard about the Panama operation (Stem Cell Institute) from local people who had gone for various reasons. Every one of them seemed well-pleased with the results they were able to get. After release from the local hospital in early March of 2011, I sent my medical records to Panama and arranged an appointment in mid-March. I traveled to Panama in a wheel chair because I was not able to walk in the airport.
Upon arrival in Panama the first day was spent drawing my blood for blood tests. The next 7 days the (umbilical cord-derived) stem cells were infused back into my body.

I found the staff to be very helpful and apparently knowledgeable. Our needs outside the clinic were well taken care of and we were very satisfied with our experience.

I steadily improved after Panama to the point where I have now resumed a normal life (at as normal as a 68 year-old guy can expect) and I am back at work. I have not had to use a nitroglycerine pill in over a year and, so far as I can tell, I have no immediate heart problems and but for the damage suffered from my earlier episodes, my heart would be in good shape. My longtime cardiologist will still see me but he believes Panama was some sort of voodoo and I have just been lucky. I continue to take a mild blood pressure medication and I do still take blood thinner. However, about 6 months after Panama my blood pressure got so low they had to cut down the strength of the medication.

What symptoms did you have before you cam for treatment?

Prior to Panama I had a series of heart attacks over a number of years and was eating nitroglycerine tablets like M&Ms.

Which treatments did you try in the U.S. first, and when did you know that you needed to find help outside of the country?

I tried every treatment available in the US of A. Had every test know to man. I mean 5 by-passes and 9 stents many not be a record but it has to be well beyond average.

Why did you choose The Stem Cell Institute in Panama for stem cell treatment?

I chose the Stem Cell Institute in Panama because several local folks had gone there and they were pleased with the results. And, I really had no place to go but the cemetery.

How were the doctors at the clinic? How were the facilities?

The only doctor I recall seeing was Dr. Paz. The facilities were at least on par with what you would find in the US and the staff was more solicitous and helpful than you would find in the US.

How are your symptoms now? How have they improved?

I have no symptoms now. (Knock on wood). I have some breathing issues but I am told that is COPD and it is not really all that bad. I could live another 100 years feeling as good as I do now.

Have you reduced or eliminated any of the medications you were taking before stem cell therapy?

I have cut way back on medication I was using prior to Panama and feel I could probably cut back some more; however, I have to give the local MD’s something to work on. And, by the way, I go to the doctor as little as possible now. Maybe twice a year at most.

How soon did you start seeing/noticing a difference? How was your overall experience?

I began to notice a difference (maybe it was just anticipation) about 6 to 8 weeks after returning from Panama. It has gotten better and better since then. Since I can move around, I have lost over 40 pounds and now am at 6 feet tall and 215 pounds, down from over 250 pounds.

How has this changed your life?

Panama did not exactly change my life. It literally gave my life back to me. I have been married to the same woman for over 50 years and we were able to become intimate again. I am out and about every day and pretty much do what I want to do and go where I want to go.

Additional Comments

Many people should know about this option. There are people dying here in the land of the free and the home of the brave each day that are in better shape than I was when I went to Panama.

And, by the way, I am not subject to flights of fancy and accepting things a thinking person might not accept. I hold a doctor of jurisprudence degree and have been a criminal defense attorney all my adult life. I am about as cynical and skeptical as you will find but I am a believer in your process.

Thank you,

Jim Parker

MS Radio – Stem cells can change our lives

On Tuesday, February 19th, 2013
at 5pm EST

Stem Cells Change Lives
Click here to Listen Online

or Call (347) 327-9317
Toll Free (877) 497-9936

Join us on Multiple Sclerosis Radio as the Director of MSstation™ Radio Judi Lecoq and her panel of nine individuals candidly share their testimonies of living with Multiple Sclerosis and their experiences after undergoing Stem Cell Treatment.

 

Judi Lecoq

I was diagnosed with Secondary Progressive Multiple Sclerosis in 1997, and began the Stem Cell Journey, with Fundraising January 2010.

SammyJo Wilkinson

Diagnosed ’95 relapsing remitting MS, secondary progressed by 2002. May 2012 I had adult stem cells in Houston, TX

Jennifer Ziegler

I was diagnosed with MS in 2004. I started thinking about Adult Stem Cell Therapy around 6 yrs. ago.

Holly Huber

Diagnosed with Multiple Sclerosis in 2004. Within months of my official diagnosis, I couldn’t walk. I was quickly facing having to live the rest of my life in a wheelchair and needing to depend on someone else 24/7 for survival. After 9 months of clinical research, in 2008 I had my first stem cell treatment.

Fiona Sparrow

Diagnosed 2005 with RRMS in 2009. Then I was told I had Malignant MS an extremely aggressive form. Only 3-5% of patients have this form. In December 29/2011 I underwent a full bone marrow transplant/stem cell transplant!

Annette Williams

Diagnosed with Relapsing Remitting MS in 2008. In 2010 it progressed to secondary progressive. I heard about stem cell therapy and begin researching about it. Following a fund raiser I went and had adult stem cells.

Carla Hickman

Diagnosed in May of 2003 with Relapsing Remitting Multiple Sclerosis. I began looking into stem cells in 2009 and went to Costa Rica in 2010.

Kane Roper

Diagnosed with Multiple Sclerosis for roughly 7 – 8 years, before receiving stem cell treatment last Christmas.

Richard Humphries

In October of 2005 after several hundred tonic seizures, I was diagnosed with Relapsing Remitting Multiple Sclerosis. By March 2007, it transition to Secondary Progressive MS. My wife and I were looking at a wheelchair. By the time I chose to have his first Stem Cell Treatments in 2008, I was completely bedridden. My Stem Cell Journey is long and varied.

Preston Walker

Diagnosed on Dec. 2001 with RRMS. I went down for ASC treatment on May 2008, June of 2009 and July of 2010.

Allogeneic and autogolous stem cell therapy combined with physical rehabilitation: A case report on a chronically injured man with quadriplegia

Allogeneic and autogolous stem cell therapy combined with physical rehabilitation - A case report on a chronically injured man with quadriplegia

Daniel Leonard in Panama

This is a research paper written by Rebecca Johnston, Daniel Leonard’s sister. She recently graduated from a Physical Therapy degree program, and wrote her Capstone paper about Daniel’s stem cell therapy treatment in Panama.

Daniel is presented anonymously in the paper, but Rebecca and Daniel have given their permission for this paper to be shared. Daniel’s ASIA scores (pre and post treatment) are in the appendix of this paper.

 

Allogeneic and autogolous stem cell therapy combined with physical rehabilitation: A case report on a chronically injured man with quadriplegia

Abstract:

Background and Purpose: Stem cell therapy for SCI is a potentially promising treatment with increasing interest. This case report describes the use of a particular stem cell therapy protocol for a patient with chronic spinal cord injury, and describes his subsequent therapy and outcomes.

Case Description: The patient is a 29-year-old male who is chronically injured from a cervical spinal injury, resulting in quadriplegia. The patient was treated with a combined protocol of intrathecal (IT) and intravaneous (IV) allogeneic MSC and CD34+ cells and IT autologous BMMC at 6 ½ years post-injury. The results track the patient’s physical therapy progress until 6 months following stem cell treatment.

Outcomes: Recovery of strength in upper extremity and lower extremity muscle groups was noted, along with a functional increase in grip strength, ability to ambulate with assistance, and a significant decrease in daily medications.
Discussion: This case supports further investigation into treatment of chronically injured SCI patients with stem cell therapy followed by physical therapy.

Manuscript word count: 4321

A few highlights:

“After the patient underwent the stem cell treatment and returned to outpatient physical therapy in his hometown clinic in the United States, his MMT scores were tested over the period of 5 months post-stem cell treatment…. The patient did not decrease in strength in any of the muscles tested, and experienced improvements in 6/13 upper extremity muscle groups, and 8/9 lower extremity muscle groups.”

“The patient also had an increase in grip strength. His grip strength was measured by his occupational therapist to be 5 lbs on the right and 25 lbs on the left at one month before his stem cell treatment. Six months later, his grip strength was measured to be 22 lbs on the right and 36 lbs on the left. The patient reported that this increase in grip strength led to functional improvements, such as being able to self-catheterize, which he was completely unable to do since his injury.”

“The patient was also able to ambulate for the first time in 5 years at approximately 4 months after finishing his treatment. He was able to ambulate in partial weight bearing with the harness and max assist of two for 40 yards at .5 MPH.”


The original post on Daniel Leonard’s blog can be found here.

Mesenchymal Stem Cells in Regenerative Medicine:Mechanisms of action, sources, and delivery options

Neil Riordan, PhD, Founder of the Stem Cell Institute in Panama City, Panama will be speaking today, Wednesday, Feb 6 at the STEMSO International Stem Cell Society 2013 Conference in Fort Lauderdale, FL.

The topic of Dr. Riordan’s discussion will be “Mesenchymal Stem Cells in Regenerative Medicine:Mechanisms of action, sources, and delivery options”

The theme for this year’s event is “Autologous Stem Cells: Who gets to decide…”

Multiple Sclerosis Radio – “Stem cells are your body’s natural healing mechanism” – Neil Riordan PhD

For anyone who missed Dr. Riordan’s talk on MS Radio yesterday, below is a link to the replay. Did you know that by age 30, 96% of the mesenchymal stem cells are gone from a person’s bone marrow? Why is MS a disease of the immune system? How can an automated machine analyze a sample of lecithin and buffer that contains no cells and show that it contains 10 million cells per ml? Tune in for these and more.

REPLAY: “Stem cells are your body’s natural healing mechanism” – Neil Riordan PhD

TODAY ON MULTIPLE SCLEROSIS RADIO!
Dr. Neil Riordan, Founder of Stem Cell Institute
Tuesday Feb 5, 2013 at 2 pm EST.

LISTEN ONLINE: Multiple Sclerosis Radio

or call in Join Us LIVE On Air
(347) 327-9317
or Toll-Free
(877) 497-9936

http://www.blogtalkradio.com/msradio/2013/02/05/stem-cells-your-bodys-natural-healing-mechanism-stem-kine-1

Liposuction method can adversely affect adipose tissue-derived stem cell yield and growth

Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure which was published in Cytotherapy (vol. 8 issue 2, 2006, pages 166-177)states that:

“Ultrasound-assisted liposuction resulted in a lower frequency of proliferating adult stem cells, as well as a longer population doubling time of adult stem cells, compared with resection…”

Those seeking adipose stem cell therapy should ask their doctor if he or she is using ultrasound assisted liposuction to collect the fat sample.

*Stem Cell Institute does not use ultrasound assisted liposuction.

Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure
M.J. Oedayrajsingh-Varma1, S.M. van Ham2, M. Knippenberg3, M.N. Helder4, J. Klein-Nulend3, T.E. Schouten5, M.J.P.F. Ritt1, F.J. van Milligen, PhD5,

1 Department of Plastic Reconstructive and Hand Surgery, VU University Medical Center, Amsterdam, the Netherlands
2 Department of Immunopathology, Sanquin Research at CLB, Amsterdam, the Netherlands
3 Department of Oral Cell Biology, ACTA-Vrije Universiteit, Amsterdam, the Netherlands
4 Department of Orthopaedic Surgery, VU University Medical Center, Amsterdam, the Netherlands
5 Department of Pathology, VU University Medical Center, Amsterdam, the Netherlands
http://dx.doi.org/10.1080/14653240600621125, How to Cite or Link Using DOI
Permissions & Reprints

Patients beware: “Point of care” fat stem cell separation and counting kits inaccurate and not US FDA approved for humans.

An informative paper by Mary Pat Moyer, PhD detailing why “same-day” fat stem cell kits that are becoming more common in doctors’ offices across the US can miscount “stem cells” by large factors leading to over estimation of stem cell counts by as much as 20 times or more.

It also states, “no complete harvest and cell isolation systems have been approved by the FDA for autologous SVF harvest for immediate use [in humans].” These are just a couple of the arguments presented that demonstrate why it’s important to process adipose tissue properly in a professional lab setting.

Morrison DG, Hunt DA, Garza I, Johnson RA, Moyer MP*. Counting and Processing Methods Impact Accuracy of Adipose Stem Cell Doses. BioProcess J, 2012; 11(4): 4-17.